Name: Optimising Terpene Synthesis with Flow Biocatalysis

Description: Sesquiterpenes are an important family of natural products that often exhibit important pharmaceutical and agricultural properties. They are biosynthesised from farnesyl diphosphate in sesquiterpene synthase catalysed reactions. Here we report the development of a highly efficient segmented flow system for the enzymatic continuous flow production of sesquiterpenes. Design of experiment (DoE) methods were used to optimise the performance of the flow biocatalysis and quantitative yields were achieved using an operationally simple but highly effective segmented flow system. All data are provided as pdf: scans were made of the paper-based experimental outputs from various measurement systems. These comprise: analysis of the reaction mixture using gas chromatography with a Flame Ionization Detector; analysis of the reaction mixture using gas chromatography with an Electron Impact Mass Spectroscopy detector; analysis of chemical compound using Nuclear Magnetic Resonance Spectroscopy. Raw NMR data are available and can be accessed with suitable software. Lab notes are provided in jpeg format.

DOI: 10.17035/d.2016.0011675544

Location: https://research.cardiff.ac.uk/converis/portal/detail/Dataset/11675544?auxfun=&lang=en_GB

Resository: https://research.cardiff.ac.uk

Start date: 2017-01-01 00:00:00 UTC

End date:

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